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pvdf membranes trans blot turbo rta transfer kit  (Bio-Rad)


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    Bio-Rad pvdf membranes trans blot turbo rta transfer kit
    Pvdf Membranes Trans Blot Turbo Rta Transfer Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pvdf+membranes+trans+blot+turbo+rta+transfer+kit/Trans-Blot+Turbo+RTA+Mini+LF+PVDF+Transfer+Kit/10__22175_slash_mmb__15701-90-7-16
    Average 96 stars, based on 270 article reviews
    pvdf membranes trans blot turbo rta transfer kit - by Bioz Stars, 2026-09
    96/100 stars

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    Western Blot:

    Article Title: Title Pending 15701
    Article Snippet: .. Proteins were transferred onto 0.45-μ polyvinylidene difluoride (PVDF) membranes (Trans-Blot Turbo RTA Transfer Kit, LF PVDF; BioRad Laboratories, Hercules, CA) using the Trans-Blot Turbo Transfer System and reagents (BioRad Laboratories) at a limited voltage of 25 V and constant 2.5 A for 12 min. Immunoblotting. .. Membranes were blocked in phosphate buffered saline (PBS)-Tween (100 mM sodium phosphate, pH 7.4; 100 mM sodium chloride; 0.1% [vol/vol] Tween-20) with 5% (wt/vol) nonfat dry milk for 1 h at room temperature, incubated overnight at 4°C in mouse monoclonal anti-rabbit troponin-T (TnT) antibody (Clone JLT-12, Sigma-Aldrich Chemical, St. Louis, MO), diluted at 1:35,000 in PBSTween, washed, and incubated for 1 h at room temperature with goat anti-mouse antibody conjugated with horseradish peroxidase (A28177) (ThermoFisher Scientific, Waltham, MA) diluted at 1:75,000 in PBS-Tween.



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